microarray hybridization solution version 2 (Danaher Inc)
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Microarray Hybridization Solution Version 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+solution/Microarray+Hybridization+Solution+v2%2E0/pmc01175033-123-5-10
Average 93 stars, based on 116 article reviews
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Hybridization:Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera. Article Snippet: .. Each slide with tissue sections was covered with 130 μL Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into Labeling:Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera. Article Snippet: .. Each slide with tissue sections was covered with 130 μL Expressing:Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Microarray:Article Title: Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells Article Snippet: Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000; 1 μg of each labeled cRNA was fragmented by adding 5 μL of 10 × Blocking Agent and 1 μL of 25 × Fragmentation Buffer and then heated at 60°C for 30 min. .. Finally, 25 μL of 2 × GE Hybridization Buffer was added to dilute the labeled cRNA; 50 μL of Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Article Title: A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera. Article Snippet: .. Each slide with tissue sections was covered with 130 μL Article Title: Microbiota-dependent activation of the myeloid calcineurin-NFAT pathway inhibits B7H3- and B7H4-dependent anti-tumor immunity in colorectal cancer. Article Snippet: In brief The mechanisms through which microsatellite-stable colorectal cancer inhibits anti-tumor immunity and resists immune checkpoint blockade are incompletely understood.. Peuker et al. describe a microbiota-dependent pathway of crosstalk between myeloid cells, T cells, and tumor cells that inhibits CD8 T cell-dependent anti-tumor immunity through the co-inhibitory proteins B7H3 and B7H4 and that is amenable to therapeutic targeting. Article Title: Snail acetylation by autophagy‐derived acetyl‐coenzyme A promotes invasion and metastasis of KRAS ‐ LKB1 co‐mutated lung cancer cells Article Snippet: .. Paraffin sections of human lung adenocarcinoma (LUAD) tissues from a tumor tissue microarray (TMA) containing 92 lung cancer samples and 88 matched normal tissues (HLugA180Su03, US Biomax, Derwood, MD, USA) were were deparaffinized in Bond dewax solution (AR9222, Leica Biosystems, Baden‐Wurttemberg, Germany) and rehydrated in absolute ethanol (1.07017, Merck, Burlington, MA, USA), and antigen retrieval was performed by boiling in 10 mmol/L sodium citrate (pH 6) for 20 min. Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into Concentration Assay:Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Staining:Article Title: Microbiota-dependent activation of the myeloid calcineurin-NFAT pathway inhibits B7H3- and B7H4-dependent anti-tumor immunity in colorectal cancer. Article Snippet: In brief The mechanisms through which microsatellite-stable colorectal cancer inhibits anti-tumor immunity and resists immune checkpoint blockade are incompletely understood.. Peuker et al. describe a microbiota-dependent pathway of crosstalk between myeloid cells, T cells, and tumor cells that inhibits CD8 T cell-dependent anti-tumor immunity through the co-inhibitory proteins B7H3 and B7H4 and that is amenable to therapeutic targeting. Immunohistochemical staining:Article Title: Microbiota-dependent activation of the myeloid calcineurin-NFAT pathway inhibits B7H3- and B7H4-dependent anti-tumor immunity in colorectal cancer. Article Snippet: In brief The mechanisms through which microsatellite-stable colorectal cancer inhibits anti-tumor immunity and resists immune checkpoint blockade are incompletely understood.. Peuker et al. describe a microbiota-dependent pathway of crosstalk between myeloid cells, T cells, and tumor cells that inhibits CD8 T cell-dependent anti-tumor immunity through the co-inhibitory proteins B7H3 and B7H4 and that is amenable to therapeutic targeting. Double Staining:Article Title: Microbiota-dependent activation of the myeloid calcineurin-NFAT pathway inhibits B7H3- and B7H4-dependent anti-tumor immunity in colorectal cancer. Article Snippet: In brief The mechanisms through which microsatellite-stable colorectal cancer inhibits anti-tumor immunity and resists immune checkpoint blockade are incompletely understood.. Peuker et al. describe a microbiota-dependent pathway of crosstalk between myeloid cells, T cells, and tumor cells that inhibits CD8 T cell-dependent anti-tumor immunity through the co-inhibitory proteins B7H3 and B7H4 and that is amenable to therapeutic targeting. Incubation:Article Title: The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria Article Snippet: .. After sectioning, the samples were treated for 20 min at 4 °C with 4% paraformaldehyde dissolved in PBS, washed in PBS for 5 min, and then incubated in 0.2 M HCl for 10 min. After washing the samples two times for 2 min in PBS, the slides were transferred into |
